FGL-HM612B | 价格

FGL-HM612B-100μg / 询价

FGL-HM612B-500μg / 询价

FGL-HM612B-500μgx2 / 询价

Biotinylated Human FGL2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human FGL2 Protein is expressed from HEK293 with His-Avi tag and Flag tag at the N-Terminus.
It contains Val205-Pro439 [Accession | Q14314].

分子量大小(Molecular Weight)

The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human FGL2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human FGL2, His Tag at 2μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).

背景(Background)

Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.

分子别名(Synonyms)

Fibroleukin; pT49; FGL2; fibrinogen-like 2;

文献(References)

Feng Y, Guo C, Wang H, Zhao L, Wang W, Wang T, Feng Y, Yuan K, Huang G. Fibrinogen-Like Protein 2 (FGL2) is a Novel Biomarker for Clinical Prediction of Human Breast Cancer. Med Sci Monit. 2020 Jul 27;26:e923531. doi: 10.12659/MSM.923531. PMID: 32716910; PMCID: PMC7409386.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.