货号价格

FGR-HM1BB-100μg / 询价

FGR-HM1BB-500μg / 询价

FGR-HM1BB-500μgx2 / 询价

Human FGFR2 beta (IIIb) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGFR2 beta (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg152-Glu378.[Accession | P21802-3]

分子量大小(Molecular Weight)

The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human FGFR2 beta (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FGFR2 beta (IIIb) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FGFR2 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).

Blocking Data

Serial dilutions of Anti-FGFR2 Antibody were added into Human FGFR2 beta (lllb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 1.74ng/ml.

SPR Data

Anti-FGFR2 Antibody, hFc Tag can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 1.98nM as determined in a SPR assay (Biacore T200).

背景(Background)

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform.

分子别名(Synonyms)

Fibroblast growth factor receptor 2;FGFR-2;KGFR;K-sam;Keratinocyte growth factor receptor;CD332;BEK;KSAM

文献(References)

(1)Katoh, Masaru. FGFR inhibitors: Effects on cancer cells, tumor microenvironment and whole-body homeostasis (Review)[J]. International Journal of Molecular Medicine, 2016.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.