货号价格

FGR-HM1BD-100μg / 询价

FGR-HM1BD-500μg / 询价

FGR-HM1BD-500μgx2 / 询价

Human FGFR2 alpha (IIIb) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGFR2 alpha (IIIb) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Arg22-Glu378.[Accession | P21802-3]

分子量大小(Molecular Weight)

The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FGFR2 alpha (IIIb), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).

Blocking Data

Serial dilutions of Anti-FGFR2 alpha (IIIb) Antibody were added into Human FGFR2 alpha (IIIb) , His Tag : Biotinylated Human FGF10, No Tag binding reactioins.

背景(Background)

Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform.

分子别名(Synonyms)

FGF R2a; FGFR2 alpha; KGFR; CD332; BBDS; BEK; BFR-1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; TK25; FLJ98662

文献(References)

(1) D'Amici S, Ceccarelli S, Vescarelli E, Romano F, Frati L, Marchese C, Angeloni A. TNFα modulates Fibroblast Growth Factor Receptor 2 gene expression through the pRB/E2F1 pathway: identification of a non-canonical E2F binding motif. PLoS One. 2013 Apr 16;8(4):e61491. doi: 10.1371/journal.pone.0061491. PMID: 23613863; PMCID: PMC3629046.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.