货号价格

FIN-HM102-100μg / 询价

FIN-HM102-500μg / 询价

FIN-HM102-500μgx2 / 询价

Human Fibronectin (32-602) Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Fibronectin (32-602) Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gln32-Leu602 [Accession | P02751-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 65.47 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Fibronectin (32-602) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Fibronectin (32-602) is greater than 95% as determined by SEC-HPLC.

背景(Background)

Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans.

分子别名(Synonyms)

FN; CIG; FN1; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; SMDCF

文献(References)

Ruoslahti E. Fibronectin. J Oral Pathol. 1981 Feb;10(1):3-13. doi: 10.1111/j.1600-0714.1981.tb01242.x. PMID: 6782207.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.