货号价格

FSF-HM218-100μg / 询价

FSF-HM218-500μg / 询价

FSF-HM218-1mg / 询价

Human GITR Ligand/TNFSF18 Trimer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GITR Ligand/TNFSF18 Trimer Protein is expressed from Expi293 with monomeric hFc tag at the N-terminal. It contains Gln50-Ser177.[Accession | Q9UNG2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 70.2 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human GITR Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human GITR Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

SPR Data

Human GITR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand (Trimer) , hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay (Biacore T200).

背景(Background)

Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept.

分子别名(Synonyms)

TNFSF18; AITRL; TL6; GITRL; GITR Ligand

文献(References)

(1)Richards DM, Marschall V, Billian-Frey K, et al. HERA-GITRL activates T cells and promotes anti-tumor efficacy independent of FcγR-binding functionality. J Immunother Cancer. 2019;7(1):191. Published 2019 Jul 19. doi:10.1186/s40425-019-0671-4

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