GAT-HM103-100μg / 询价
GAT-HM103-500μg / 询价
GAT-HM103-500μgx2 / 询价
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Human GARP (S138G) &Latent TGF Beta 1 Complex Protein
Recombinant Human GARP(S138G)&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628 (GARP(S138G)) & Leu30-Ser390 (Latent TGF Beta 1) [Accession | Q14392(GARP(S138G))&P01137(Latent TGF beta 1)].
The protein has a predicted MW of 70.3 kDa (GARP(S138G))&41.4 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 75-80 kDa (GARP(S138G))&15 kDa&42-48 kDa (Latent TGF Beta 1) based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human GARP (S138G) &Latent TGF Beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GARP (S138G) &Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1
(1) Stockis J, Dedobbeleer O, Lucas S. Role of GARP in the activation of latent TGF-β1. Mol Biosyst. 2017 Sep 26;13(10):1925-1935.
(2) Metelli A, Salem M, Wallace CH, Wu BX, Li A, Li X, Li Z. Immunoregulatory functions and the therapeutic implications of GARP-TGF-β in inflammation and cancer. J Hematol Oncol. 2018 Feb 20;11(1):24.
