GAT-HM401-100μg / 询价
GAT-HM401-500μg / 询价
GAT-HM401-500μgx2 / 询价
- 客服电话
- 技术支持
Human GARP&Latent TGF Beta 1 Complex Protein
Recombinant Human GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1) [Accession | Q14392(GARP)&P01137(Latent TGF beta 1)].
The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 72-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human GARP&Latent TGF Beta 1 Complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human GARP&Latent TGF Beta 1 Complex is greater than 95% as determined by SEC-HPLC.
Immobilized Human GARP&Latent TGF Beta 1 Complex, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Anti-GARP&TGF beta 1 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA.
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1
(1) Stockis J, Dedobbeleer O, Lucas S. Role of GARP in the activation of latent TGF-β1. Mol Biosyst. 2017 Sep 26;13(10):1925-1935.
(2) Metelli A, Salem M, Wallace CH, Wu BX, Li A, Li X, Li Z. Immunoregulatory functions and the therapeutic implications of GARP-TGF-β in inflammation and cancer. J Hematol Oncol. 2018 Feb 20;11(1):24.
