GAT-RM401-100μg / 询价
GAT-RM401-500μg / 询价
GAT-RM401-500μgx2 / 询价
Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Mouse&Human Chimeric Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Rhesus macaque Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Mouse Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
表达区间及表达系统(Source)
|
Recombinant Rat GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Ile18-Asn628(GARP)&Leu30-Arg278(Latent TGF Beta 1) [Accession | D3ZVD5(GARP)&P17246(Latent TGF Beta)]. |
分子量大小(Molecular Weight)
|
The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result. |
纯度(Purity)
|
> 95% as determined by Bis-Tris PAGE |
内毒素(Endotoxin)
|
Less than 1EU per μg by the LAL method. |
制剂(Formulation)
|
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution)
|
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储(Storage)
|
-20 to -80°C for 24 months as supplied from date of receipt. |
Recombinant Rat GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Ile18-Asn628(GARP)&Leu30-Arg278(Latent TGF Beta 1) [Accession | D3ZVD5(GARP)&P17246(Latent TGF Beta)].
The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1
(1) Stockis J, Dedobbeleer O, Lucas S. Role of GARP in the activation of latent TGF-β1. Mol Biosyst. 2017 Sep 26;13(10):1925-1935.
(2) Metelli A, Salem M, Wallace CH, Wu BX, Li A, Li X, Li Z. Immunoregulatory functions and the therapeutic implications of GARP-TGF-β in inflammation and cancer. J Hematol Oncol. 2018 Feb 20;11(1):24.
GAT-RM401-100μg / 询价
GAT-RM401-500μg / 询价
GAT-RM401-500μgx2 / 询价
Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Mouse&Human Chimeric Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Rhesus macaque Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
Mouse Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein
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