GDF-HM008-100μg / 询价
GDF-HM008-500μg / 询价
GDF-HM008-500μgx2 / 询价
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Human/Cynomolgus Activin RIIB/ACVR2B Protein
Human/Cynomolgus Activin RIIB/ACVR2B Protein
Human/Cynomolgus Activin RIIA/ACVR2A Protein
Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein
Biotinylated Human/Cynomolgus Activin RIIB/ACVR2B Protein
Human/Cynomolgus Activin RIIA/ACVR2A Protein
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Human/Mouse/Rat GDF-8 Protein
Recombinant Human/Mouse/Rat GDF-8 Protein is expressed from HEK293 without tag.It contains Asp267-Ser375 [Accession | O14793].
The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in 4 mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4 mM HCl.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human/Mouse/Rat GDF-8 on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%.
Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is <30 ng/ml (QC Test).
Immobilized Human/Mouse/Rat GDF-8, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 0.13 μg/ml determined by ELISA (QC Test).
Immobilized Human/Mouse/Rat GDF-8, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8 ng/ml determined by ELISA.
Immobilized Human GDF-8, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9 ng/ml determined by ELISA.
Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).
Biotinylated Activin RIIB, His Tag captured on CM5 Chip via Streptavidin can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Human/Mouse/Rat GDF-8, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).
Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Myostatin; GDF8; Mstn; GDF-8; Myostatin
Walker RG, McCoy JC, Czepnik M, Mills MJ, Hagg A, Walton KL, Cotton TR, Hyvönen M, Lee RT, Gregorevic P, Harrison CA, Thompson TB. Molecular characterization of latent GDF8 reveals mechanisms of activation. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E866-E875. doi: 10.1073/pnas.1714622115. Epub 2018 Jan 18. PMID: 29348202; PMCID: PMC5798348.
