Biotinylated Human GPRC5D Protein-VLP
Recombinant Biotinylated Human GPRC5D Protein-VLP is expressed from HEK293. It contains Met1-Val345.[Accession | Q9NZD1-1]
The target protein has a predicted MW of 39.6 kDa.
Less than 1EU per μl by the LAL method.
> 95% as determined by HPLC
Supplied as 0.22μm filtered solution in PBS, 300mM L-Arginine (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC.
Immobilized Human CD3E&CD3D, hFc Tag at 1μg/ml (100μl/well) on the plate. Add serial dilutions of Anti-CD3×GPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5ng/ml determined by ELISA.
Biotinylated Human GPRC5D VLP captured on SA Chip can bind Anti-GPRC5D antibody, hFc Tag with an affinity constant of 0.30 nM as determined in SPR assay (Biacore T200) (QC Test).
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
(1) Smith EL, Harrington K, Staehr M, Masakayan R, Jones J, Long TJ, Ng KY, Ghoddusi M, Purdon TJ, Wang X, Do T, Pham MT, Brown JM, De Larrea CF, Olson E, Peguero E, Wang P, Liu H, Xu Y, Garrett-Thomson SC, Almo SC, Wendel HG, Riviere I, Liu C, Sather B, Brentjens RJ. GPRC5D is a target for the immunotherapy of multiple myeloma with rationally designed CAR T cells. Sci Transl Med. 2019 Mar 27;11(485):eaau7746. doi: 10.1126/scitranslmed.aau7746. PMID: 30918115; PMCID: PMC7508042.
