Human GITR/TNFRSF18 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GITR/TNFRSF18 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln26-Glu161.[Accession | Q9Y5U5-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%

SEC-HPLC

The purity of Human GITR is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GITR, His Tag at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA.

ELISA Data

Immobilized Human GITR, His Tag at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.

SPR Data

Human GITR, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human GITR Ligand Trimer, hFc Tag with an affinity constant of 4.76 nM as determined in SPR assay (Biacore T200).

背景(Background)

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

分子别名(Synonyms)

CD357; GITR; GITR-D; TNFRSF18; AITR

文献(References)

(1)Knee D A , Hewes B , Brogdon J L. Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10.

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