Human GITR/TNFRSF18 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human GITR/TNFRSF18 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gln26-Glu161 [Accession | Q9Y5U5-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human GITR is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GITR Ligand Trimer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human GITR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA.

背景(Background)

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

分子别名(Synonyms)

CD357; GITR; GITR-D; TNFRSF18; AITR

文献(References)

(1)Knee D A , Hewes B , Brogdon J L. Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.