货号价格

HLG-RM41C-100μg / 询价

HLG-RM41C-500μg / 询价

HLG-RM41C-500μgx2 / 询价

Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305 (HLA-G), Ile21-Met119 (B2M) and RIIPRHLQL peptide.[Accession | O02948(HLA-G)&Q6V7J5(B2M)&RIIPRHLQL]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Rhesus macaque LILRB1, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA (QC Test).

SPR Data

Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G&B2M&Peptide (RIIPRHLQL) Monomer, His Tag with an affinity constant of 27.18 nM as determined in SPR assay (Biacore T200).

背景(Background)

HLA-G is a molecule that was first known to confer protection to the fetus from destruction by the immune system of its mother, thus critically contributing to fetal-maternal tolerance. The first functional finding constituted the basis for HLA-G research and can be summarized as such: HLA-G, membrane-bound or soluble, strongly binds its inhibitory receptors on immune cells (NK, T, B, monocytes/dendritic cells), inhibits the functions of these effectors, and so induces immune inhibition.

分子别名(Synonyms)

HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G

文献(References)

(1) Krijgsman D, Roelands J, Hendrickx W, Bedognetti D, Kuppen PJK. HLA-G: A New Immune Checkpoint in Cancer? Int J Mol Sci. 2020 Jun 25;21(12):4528. doi: 10.3390/ijms21124528. PMID: 32630545; PMCID: PMC7350262.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.