IFN-HM40GB | 价格

IFN-HM40GB-100μg / 询价

IFN-HM40GB-500μg / 询价

IFN-HM40GB-500μgx2 / 询价

Biotinylated Human IFN gamma/IFNG Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human IFN gamma/IFNG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Gln24-Gly161 [Accession | P01579].

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 25-40 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human IFN gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Biotinylated Human IFN gamma, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human IFNGR1, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.

SEC-HPLC

The purity of Biotinylated Human IFN gamma is greater than 95% as determined by SEC-HPLC.

背景(Background)

Interferon-gamma (IFN gamma) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. The absence of IFN gamma production or cellular responsiveness in humans and experimental animals significantly predisposes the host to microbial infection, a result that validates the physiologic importance of this cytokine in preventing infectious disease.

分子别名(Synonyms)

Interferon-gamma; Interferon-γ; interferon; gamma; IFG; IFI; IFN gamma; IFNγ; IFN γ

文献(References)

(1)Ikeda H, Old LJ, Schreiber RD. The roles of IFN gamma in protection against tumor development and cancer immunoediting. Cytokine Growth Factor Rev. 2002;13(2):95-109. doi:10.1016/s1359-6101(01)00038-7.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.