IGG-MM02A-100μg / 询价
IGG-MM02A-500μg / 询价
IGG-MM02A-500μgx2 / 询价
Mouse IgG2a Fc Protein
Recombinant Mouse IgG2a Fc Protein is expressed from HEK293 without tag.It contains Glu98-Lys330 [Accession | P01863].
The protein has a predicted MW of 26.38 kDa. Due to glycosylation, the protein migrates to 33-38 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse IgG2a Fc on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse IgG2a Fc is greater than 95% as determined by SEC-HPLC.
Mouse FcRn, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse IgG2a Fc, No Tag with an affinity constant of 2.72 nM as determined in SPR assay (Biacore T200).
Mouse IgG2a is one of the subtypes of murine immunoglobulin IgG which mediates immune responses to carbohydrate or polysaccharide antigens. The Fc region of IgG2a can bind to FcRn to prolong its half-life.
IgG2a; IgG2a-Fc; Igh-1; Igh 1; Immunoglobulin G2A Fc; G2A Fc
[1] Jacobson BA, Rothstein TL, Marshak-Rothstein A. Unique site of IgG2a and rheumatoid factor production in MRL/lpr mice. Immunol Rev. 1997 Apr;156:103-10.
[2] Majlessi L, Rujithamkul N, Bordenave G. Mechanisms of T-cell-induced allotypic suppression of mouse IgG2a(b) and of tolerance acquisition to this allotype. Res Immunol. 1995 May-Jun;146(4-5):213-24.
[3] Martin RM, Brady JL, Lew AM. The need for IgG2c specific antiserum when isotyping antibodies from C57BL/6 and NOD mice. J Immunol Methods. 1998 Mar 15;212(2):187-92.
