货号价格

IL1-HE00A-100μg / 询价

IL1-HE00A-500μg / 询价

IL1-HE00A-500μgx2 / 询价

Human IL-1 alpha/IL-1A Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-1 alpha/IL-1A Protein is expressed from E.coli without tag.
It contains Ser113-Ala271 [Accession | NP_000566.3].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 0.05 EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-1 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.

SPR Data

Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).

背景(Background)

The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.

分子别名(Synonyms)

Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1

文献(References)

(1) Malik A, Kanneganti TD. Function and regulation of IL-1α in inflammatory diseases and cancer. Immunol Rev. 2018 Jan;281(1):124-137. doi: 10.1111/imr.12615. PMID: 29247991; PMCID: PMC5739076.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.