IL1-HM2R2-100μg / 询价
IL1-HM2R2-500μg / 询价
IL1-HM2R2-500μgx2 / 询价
Human IL-1R2/IL-1 RII/CD121b Protein
Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe14-Glu343 [Accession | NP_004624.1].
The protein has a predicted MW of 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-1R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA (QC Test).
Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
The IL-1 type II receptor (decoy RII) is a nonsignaling molecule the only established function of which is to capture IL-1 and prevent it from interacting with signaling receptor. The decoy RII is released in a regulated way from the cell surface.
IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β
(1) Orlando S, Sironi M, Bianchi G, Drummond AH, Boraschi D, Yabes D, Mantovani A. Role of metalloproteases in the release of the IL-1 type II decoy receptor. J Biol Chem. 1997 Dec 12;272(50):31764-9. doi: 10.1074/jbc.272.50.31764. PMID: 9395521.
