IL2-CM123-100μg / 询价
IL2-CM123-500μg / 询价
IL2-CM123-500μgx2 / 询价
Cynomolgus IL-23 alpha&IL-12 beta Protein
Recombinant Cynomolgus IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-terminus.It contains Val22-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta) [Accession | G7PIH8(IL-23 alpha)&XP_045250400.1(IL-12 beta)].
The protein has a predicted MW of 19.52 kDa (IL-23 alpha) and 34.76 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 20-25 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus IL-23 alpha&IL-12 beta, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).
Human IL-23R, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 4.05 nM as determined in SPR assay (Biacore T200).
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β
(1)Ma C, Panaccione R, Khanna R, Feagan BG, Jairath V. IL12/23 or selective IL23 inhibition for the management of moderate-to-severe Crohn's disease? Best Pract Res Clin Gastroenterol. 2019 Feb-Apr;38-39:101604. doi: 10.1016/j.bpg.2019.02.006. Epub 2019 Feb 19. PMID: 31327402.
