IL2-CM1AB-100μg / 询价
IL2-CM1AB-500μg / 询价
IL2-CM1AB-500μgx2 / 询价
Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein
Recombinant Cynomolgus IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val22-Pro189(Cynomolgus IL-23 alpha)&Met23-Ser335(Mouse IL-12 beta) [Accession | A0A2K5TLV4-1(Cynomolgus IL-23 alpha)&P43432-1(Mouse IL-12 beta)].
The protein has a predicted MW of 19.5 (Cynomolgus IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 57.5ng/ml determined by ELISA (QC Test).
Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200).
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β
(1) Esch A, Masiarz A, Mossner S, Moll JM, Grötzinger J, Schröder J, Scheller J, Floss DM. Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors. J Biol Chem. 2020 Jul 24;295(30):10478-10492. doi: 10.1074/jbc.RA120.013935. Epub 2020 Jun 9. PMID: 32518162; PMCID: PMC7383385.
