IL2-MM1AB | 价格

IL2-MM1AB-100μg / 询价

IL2-MM1AB-500μg / 询价

IL2-MM1AB-500μgx2 / 询价

Mouse IL-23 alpha&IL-12 beta Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the N-Terminus.
It contains Val22-Ala196(IL-23 alpha)&Met23-Ser335(IL-12 beta) [Accession | Q9EQ14-1(IL-23 alpha)&P43432-1(IL-12 beta)].

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.8 (Mouse IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa. Due to glycosylation, the protein migrates to 22 (Mouse IL-23 alpha)&45-50 (Mouse IL-12 beta) kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.

SPR Data

Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.31 nM as determined in SPR assay (Biacore T200).

背景(Background)

Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

分子别名(Synonyms)

IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β

文献(References)

(1) Esch A, Masiarz A, Mossner S, Moll JM, Grötzinger J, Schröder J, Scheller J, Floss DM. Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors. J Biol Chem. 2020 Jul 24;295(30):10478-10492. doi: 10.1074/jbc.RA120.013935. Epub 2020 Jun 9. PMID: 32518162; PMCID: PMC7383385.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.