Mouse IL-2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.
It contains Ala21-Gln169 [Accession | P04351-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per ug by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse IL-2 is greater than 95% as determined by SEC-HPLC.

SPR Data

Mouse IL-2 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-2, His Tag with an affinity constant of 30.49 nM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 1-5 ng/mL.

背景(Background)

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

分子别名(Synonyms)

IL2; T-cell growth factor; TCGF; Aldesleukin

文献(References)

(1)Sim G C , Martin-Orozco N , Jin L , et al. IL-2 therapy promotes suppressive ICOS Treg expansion in melanoma patients[J]. The Journal of clinical investigation, 2013, 124(1).

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.