货号价格

IL3-HE003-100μg / 询价

IL3-HE003-500μg / 询价

IL3-HE003-500μgx2 / 询价

Human IL-3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-3 Protein is expressed from E.coli without tag. It contains Ala20-Phe152.[Accession | AAC08706]

分子量大小(Molecular Weight)

The protein has a predicted MW of 15.09 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.

Cell Based Assay

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.3 ng/mL.

背景(Background)

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

分子别名(Synonyms)

IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF

文献(References)

(1)Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis[J]. Science, 2015, 347(6227):1260-1265.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.