货号价格

IL3-HM2RA-100μg / 询价

IL3-HM2RA-500μg / 询价

IL3-HM2RA-500μgx2 / 询价

Human IL-3 R alpha/CD123 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Thr19-Arg305.[Accession | P26951-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 59.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 90% as determined by Tris-Bis PAGE
> 90% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

SEC-HPLC

The purity of Human IL-3 R alpha is greater than 90% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-3, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46μg/ml determined by ELISA.

背景(Background)

Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

分子别名(Synonyms)

CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha

文献(References)

(1)Ugo Testa, Elvira Pelosi, Arthur Frankel.CD 123 is a membrane biomarker and a therapeutic target in hematologic malignancies.Biomark Res. 2014; 2: 4

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.