IL4-HM401-100μg / 询价
IL4-HM401-500μg / 询价
IL4-HM401-500μgx2 / 询价
Human IL-4 Protein
Recombinant Human IL-4 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153 [Accession | P05112].
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human IL-4 at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is <0.2 ng/mL.
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, and this cytokine is a ligand for interleukin 4 receptor.
IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
(1) Ghoreschi K, Thomas P, Breit S, et al. Interleukin-4 therapy of psoriasis induces Th2 responses and improves human autoimmune disease[J]. Nature Medicine, 2002, 9(1):40-46.
