IL6-HE001-100μg / 询价

IL6-HE001-500μg / 询价

IL6-HE001-1mg / 询价

Human IL-6 Protein

产品信息(Product Info)

Recombinant Human IL-6 is expressed from E.coli without tag. It contains Val30-Met212.[Accession | P05231]

分子量大小(Molecular Weight)

The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result.


Less than 1EU per μg by the LAL method.


> 95% as determined by Tris-Bis PAGE
> 90% as determined by HPLC


Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.


Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.


-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human IL-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.


The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.


Immobilized Human IL-6 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA.

SPR Data

Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

Cell Based Assay

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC Test).


IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.


IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2


(1)Gabay C. Interleukin-6 and chronic inflammation[J]. Arthritis Research & Therapy, 2006, 8.