IL7-HE001-100μg / 询价
IL7-HE001-500μg / 询价
IL7-HE001-1mg / 询价
Human IL-7 Protein
Recombinant Human IL-7 Protein is expressed from E.coli without tag. It contains Asp26-His177.[Accession | P13232]
The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8).
-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA.
Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 4.18 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC tested).
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
(1)Park JH, Waickman AT, Reynolds J, Castro M, Molina-París C. IL7 receptor signaling in T cells: A mathematical modeling perspective. Wiley Interdiscip Rev Syst Biol Med. 2019;11(5):e1447. doi:10.1002/wsbm.1447
