IL7-HM1RA | 价格

IL7-HM1RA-100μg / 询价

IL7-HM1RA-500μg / 询价

IL7-HM1RA-500μgx2 / 询价

Human IL-7 R alpha/CD127 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-7 R alpha/CD127 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Glu21-Gly236 [Accession | P16871-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-7 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-7 R alpha is greater than 95% as determined by SEC-HPLC.

SPR Data

Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200).

背景(Background)

Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.

分子别名(Synonyms)

IL-17R alpha; IL7Ra; CD127; CDW127; ILRA

文献(References)

(1)Oliveira ML, Akkapeddi P, Ribeiro D, Melão A, Barata JT. IL-7R-mediated signaling in T-cell acute lymphoblastic leukemia: An update. Adv Biol Regul. 2019 Jan;71:88-96. doi: 10.1016/j.jbior.2018.09.012. Epub 2018 Sep 19. PMID: 30249539; PMCID: PMC6386770.D

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.