ILB-HM4AB | 价格

ILB-HM4AB-100μg / 询价

ILB-HM4AB-500μg / 询价

ILB-HM4AB-500μgx2 / 询价

Human IL-23 alpha&Mouse IL-12 beta Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta) [Accession | Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta)].

分子量大小(Molecular Weight)

The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15μg/ml determined by ELISA.

背景(Background)

Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

分子别名(Synonyms)

IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β

文献(References)

(1) Esch A, Masiarz A, Mossner S, Moll JM, Grötzinger J, Schröder J, Scheller J, Floss DM. Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors. J Biol Chem. 2020 Jul 24;295(30):10478-10492. doi: 10.1074/jbc.RA120.013935. Epub 2020 Jun 9. PMID: 32518162; PMCID: PMC7383385.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.