ILR-HM127 | 价格

ILR-HM127-100μg / 询价

ILR-HM127-500μg / 询价

ILR-HM127-500μgx2 / 询价

Human IL-27RA/TCCR Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gly34-Lys516 [Accession | Q6UWB1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).

SPR Data

Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200).

背景(Background)

A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects.

分子别名(Synonyms)

IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA

文献(References)

(1)Shats O, Goldner W, Feng J, Sherman A, Smith RB, Sherman S. Thyroid Cancer and Tumor Collaborative Registry (TCCR). Cancer Inform. 2016 May 3;15:73-9. doi: 10.4137/CIN.S32470. PMID: 27168721; PMCID: PMC4856228.