ITG-HM4V5B-100μg / 询价
ITG-HM4V5B-500μg / 询价
ITG-HM4V5B-500μgx2 / 询价
- 客服电话
- 技术支持
Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein
Recombinant Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail [Accession | P06756-1(ITGAV)&P18084(ITGB5)].
The protein has a predicted MW of 114 kDa (ITGAV) & 81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human ITGAV&ITGB5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ITGAV&ITGB5 is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human ITGAV&ITGB5, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.
Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C.
αVβ5; Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5;Integrin alpha V β 5; β5
(1)Karasu Ebru E, Nilsson Bo B, Köhl Jörg J, Lambris John D JDFriedlander M , Theesfeld C L , Sugita M , et al. Involvement of integrins alpha v beta 3 and alpha v beta 5 in ocular neovascular diseases.[J]. Proceedings of the National Academy of Sciences, 1996, 93(18):9764-9769.
