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| 来源（Source） | S. pyogenes |
| 表达系统（Express System） | E.coli |
| 浓度（Concentration） | 10mg/ml |
| 产品性状（Product Character） | 溶液澄清，无悬浊物，无沉淀或杂质 |
| 纯度（Purity）（Tris-Bis PAGE） | 不低于95% |
| 纯度（Purity）（SEC-HPLC） | 不低于95% |
| 纯度（Purity）（RP-HPLC） | 不低于95% |
| 储存缓冲液（Storage Buffer） | 30mM Tris-HCl, 0.3M NaCl, 0.1mM EDTA, 50% Glycerol，pH7.4 |
| 运输/保存方法（Shipping and Storage Method） | 干冰运输， -20±5℃保存，避免反复冻融 |
| 产品应用（Product Application） | 参与细胞与基因治疗药物（如造血干细胞、T细胞等）的基因修饰 |
| 浓度为9.5-12.5mg/ml | 纯度不低于95% | 细菌内毒素不高于10EU/mg |
| 体外切割效率>85% | DNA酶残留不高于LOD | RNA酶残留不高于LOD |
| 宿主蛋白残留不高于100ng/ml | 宿主DNA残留不高于200ng/ml | 支原体未检出 |
The purity of Kactus Cas9 detected by Bis-Tris PAGE is higher than 95%.
The purity of Kactus Cas9 detected by RP-HPLC is higher than 95%.
Cas9 cuts the substrate DNA standard through the in vitro cutting experiment. The result of the figure shows that the cleavage activity of Kactus Cas9 is equivalent to that of other brands.
Cas9 is used for gene knockout in the 293T cell line. The results show that Kactus Cas9 has the same knockout efficiency in cells as foreign brand.
Recombinant Streptococcus pyogenes Cas9 nuclease, purified from E.coli, is a powerful tool for genome editing. Besides, the nuclease can also be applied to the field of molecular diagnosis. This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification. In addition, it is manufactured in accordance with GMP requirements and meets GMP quality standards.
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