货号价格

KACTUS-CAS9-GMP-3 mg / 询价

Cas9 Nuclease, GMP grade DMF Filed

COA DS
COA DS
产品描述(Product Description)

GMP 级别 Cas9 核酸酶克隆自野生型酿脓链球菌 Streptococcus pyogenes, 可实现对造血干细胞、T 细胞等的基因修饰,也可以应用于分子诊断领域,实现对病原体的高灵敏度、高特异性检测。本品是基于公司独特的创新型功能重组蛋白生产平台 SAMSTM 设计,经过野生型 spCas9 基因的密码子优化、核定位信号 (NLS) 的设计和大肠杆菌表达体系与纯化工艺的优化,并按照 GMP 要求生产得到。

产品信息(Product Info)

表达系统(Source)

E.coli;Asp2-Asp1368; [Accession | Q99ZW2]

种属(Species)

Streptococcus pyogenes

分子量大小(Molecular Weight)

The protein has a predicted MW of 163 kDa.

浓度(Concentration)

10 mg/ml

纯度(Purity)(SEC-HPLC)

≥95%

纯度(Purity)(RP-HPLC)

≥95%

内毒素(Endotoxin)

≤10 EU/mg

储存缓冲液(Storage Buffer)

30 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4

运输/保存条件(Shipping and Storage Condition)

干冰运输, -20 ± 5℃保存,避免反复冻融

产品应用(Product Applications)

参与细胞与基因治疗药物(如造血干细胞、T 细胞等)的基因修饰。

质量要求(Quality Requirement)
浓度符合规定
鉴别符合规定
纯度(RP-HPLC)≥95%
纯度(SEC-HPLC)≥95%
细菌内毒素≤10 EU/mg
体外切割效率≥85%
DNA酶残阴性
RNA酶残留阴性
宿主蛋白残留≤100 ng/ml
宿主DNA残留≤3 ng/ml
镍盐残留阴性
无菌检测未检出
产品数据(Assay Data)
SEC-HPLC

The purity of Cas9 Nuclease detected by SEC-HPLC is greater than 95%.

RP-HPLC

The purity of Cas9 Nuclease detected by RP-HPLC is greater than 95%.

In vitro cleavage activity

Three batches of Kactus Cas9 Nuclease cleaved substrate DNA standards in vitro. Results show that the cleavage activity are similar across all batches, indicating stable activity across batches.

Gene knockout efficiency

Gene knockout efficiency of Cas9 Nuclease analyzed in 293T, Jurkat and T cells using TIDE analysis. Results show editing efficacy are comparable to the competitor across all three cell types.