KGF-HE101 | 价格

KGF-HE101-100μg / 询价

KGF-HE101-500μg / 询价

KGF-HE101-500μgx2 / 询价

Human FGF-7/KGF Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGF-7/KGF Protein is expressed from E.coli with His tag at the N-Terminus.
It contains Cys32-Thr194 [Accession | P21781-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.1 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50mM Tris, 250mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human FGF-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FGF-7, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIb), hFc Tag with the EC50 of 1.22μg/ml determined by ELISA (QC Test).

SPR Data

Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).

背景(Background)

The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.

分子别名(Synonyms)

FGF-7; HBGF-7; KGF; FGF7

文献(References)

(1) Qiao J, Uzzo R, Obara-Ishihara T, Degenstein L, Fuchs E, Herzlinger D. FGF-7 modulates ureteric bud growth and nephron number in the developing kidney. Development. 1999 Feb;126(3):547-54. PMID: 9876183.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.