LAP-HM4B1B-100μg / 询价
LAP-HM4B1B-500μg / 询价
LAP-HM4B1B-500μgx2 / 询价
Biotinylated Human LAP (TGF beta 1) Protein
Recombinant Biotinylated Human LAP (TGF beta 1) Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu30-Arg278(C33S) [Accession | P01137].
The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human LAP (TGF beta 1) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human ITGAV&ITGB6, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86μg/ml determined by ELISA.
Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1
(1)Robertson I B , Rifkin D B. Regulation of the Bioavailability of TGF-β and TGF-β-Related Proteins[J]. Cold Spring Harbor Perspectives in Biology, 2016, 8(6):a021907.
