LCS-VM1S1-100μg / 询价
LCS-VM1S1-500μg / 询价
LCS-VM1S1-500μgx2 / 询价
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SARS-CoV-2 Spike S1 (Lambda C.37) Protein
Recombinant SARS-CoV-2 Spike S1 (Lambda C.37) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln14-Arg683(G75V, T76I, R246N, 247-253 del, L452Q, F490S, D614G) [Accession | QHD43416.1].
The protein has a predicted MW of 75.28 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-CoV-2 Spike S1 (Lambda C.37) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-CoV-2 Spike S1 (Lambda C.37) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-CoV-2 Spike S1 (Lambda C.37) , His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
(1)Belouzard S , Chu V C , Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.
