LGT-HM131-100μg / 询价
LGT-HM131-500μg / 询价
LGT-HM131-500μgx2 / 询价
Human LIGHT/TNFSF14 Protein
Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Asp74-Val240.[Accession | O43557-1]
The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human LIGHT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human LIGHT, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).
Immobilized Human LIGHT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 22.9ng/ml determined by ELISA.
LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
CD258; TNFSF14; HVEM-L; LIGHT; LTg; TR2
(1)Granger S W, Rickert S. LIGHT-HVEM signaling and the regulation of T cell-mediated immunity[J]. Cytokine Growth Factor Rev, 2003, 14(3):289-296.
