LIF-HM001-100μg / 询价
LIF-HM001-500μg / 询价
LIF-HM001-500μgx2 / 询价
Human LIF Protein
Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202 [Accession | P15018-1].
The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 0.1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.17μg/ml determined by ELISA (QC Test).
Human LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LIF is greater than 95% as determined by SEC-HPLC.
Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50 for this effect is 1-5 ng/mL.
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
LIF; HILDA; MLPLI; CDF; DIA
(1) Nicola NA, Babon JJ. Leukemia inhibitory factor (LIF). Cytokine Growth Factor Rev. 2015 Oct;26(5):533-44. doi: 10.1016/j.cytogfr.2015.07.001. Epub 2015 Jul 4. PMID: 26187859; PMCID: PMC4581962.
