货号价格

LIL-HM4A1-100μg / 询价

LIL-HM4A1-500μg / 询价

LIL-HM4A1-500μgx2 / 询价

Human LILRA1/CD85i/LIR-6 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human LILRA1/CD85i/LIR-6 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro17-Asn461.[Accession | O75019-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 51.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human LILRA1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human LILRA1 is greater than 95% as determined by SEC-HPLC.

背景(Background)

LILRA1, also known as CD85i and LIR-6, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA1 consists of a 445 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail. LILRA1 may act as receptor for class I MHC antigens.

分子别名(Synonyms)

CD85i; LILRA1; LIR6; LIR-6; LIR6MGC126563

文献(References)

(1)The MHC class I‐LILRB1 signalling axis as a promising target in cancer therapy[J]. Scandinavian Journal of Immunology, 2019.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.