货号价格

LIL-HM4B2-100μg / 询价

LIL-HM4B2-500μg / 询价

LIL-HM4B2-1mg / 询价

Human LILRB2/CD85d/ILT4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln22-His458.[Accession | Q8N423-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human LILRB2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human LILRB2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.

Blocking Data

Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2, His Tag : Biotinylated HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 75.3ng/ml.

背景(Background)

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.

分子别名(Synonyms)

CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10

文献(References)

(1)Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity.J Clin Invest. 2018;128(12):5647-5662.

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