LMN-HM511-100μg / 询价
LMN-HM511-100μg x 5 / 询价
LMN-HM511-100μg x 10 / 询价
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Recombinant protein expressed in a human cell line.
≥95%
0.1 mg/ml
< EU/ml
Negative
PBS, 10% glycerol, pH7.4
-20°C to -80°C, stable for at least 2 years from date of receipt.
3 months at 2-8°C after thaw.
Laminin 511, also known as LN511 or Laminin-10, is a heterotrimeric glycoprotein composed of one alpha 5, one beta 1, and one gamma 1 chain. It is a potent adhesive and pro-migratory substrate that plays a crucial role in stem cell culture for its ability to support the self-renewal and maintenance of pluripotency in stem cells, particularly in the culture of embryonic stem cells (ES) and induced pluripotent stem cells (iPS). Laminin 511 is often used as a component of the extracellular matrix to replace traditional animal-derived matrices like Matrigel, offering a more defined and controlled culture system for research and therapeutic applications in regenerative medicine.
• Ideal for feeder-free cell culture
• Enables the passaging of single cells
• Keeps mouse stem cells in pluripotent state for months without the need of feeder cells or LIF
• Provides superior adhesion of human ES and iPS cells
• Compatible across a wide variety of cell types
(1) Pouliot N, Kusuma N. Laminin-511: a multi-functional adhesion protein regulating cell migration, tumor invasion and metastasis. Cell Adh Migr. 2013 Jan-Feb;7(1):142-9. doi: 10.4161/cam.22125.
(2) Zhao C, Zhou Q, Duan H, Wang X, Jia Y, Gong Y, Li W, Dong C, Li Z, Shi W. Laminin 511 Precoating Promotes the Functional Recovery of Transplanted Corneal Endothelial Cells. Tissue Eng Part A. 2020 Nov;26(21-22):1158-1168. doi: 10.1089/ten.TEA.2020.0047.
(3) Takizawa M, Arimori T, Taniguchi Y, Kitago Y, Yamashita E, Takagi J, Sekiguchi K. Mechanistic basis for the recognition of laminin-511 by α6β1 integrin. Sci Adv. 2017 Sep 1;3(9):e1701497. doi: 10.1126/sciadv.1701497.
(4) Laminins in Cellular Differentiation. Trends Cell Biol. 2019 Dec;29(12):987-1000.
