MHC-HM421F | 价格

MHC-HM421F-100μg / 询价

MHC-HM421F-500μg / 询价

MHC-HM421F-500μgx2 / 询价

FITC-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide [Accession | AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGAVGVGK].

分子量大小(Molecular Weight)

The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 51-65 kDa based on Bis-Tris PAGE result.

波长(Wavelength)

Excitation Wavelength: 490 nm

Emission Wavelength: 520 nm

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

FITC-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of FITC-Labeled Human HLA-A*11:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer is greater than 95% as determined by SEC-HPLC.

背景(Background)

Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.

分子别名(Synonyms)

MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD

文献(References)

(1) Wu HZ, Xiao JQ, Xiao SS, Cheng Y. KRAS: A Promising Therapeutic Target for Cancer Treatment. Curr Top Med Chem. 2019;19(23):2081-2097. doi: 10.2174/1568026619666190905164144. PMID: 31486755.