MHC-HM43RTC-100μg / 询价
MHC-HM43RTC-500μg / 询价
MHC-HM43RTC-500μgx2 / 询价
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APC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein
Recombinant APC-equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gly25-Thr305 (HLA-A*02:01) and Ile21-Met119 (B2M).[Accession | A0A140T913(HLA-A*02:01)&P61769(B2M)]
The protein has a predicted MW of 293.2 kDa.
Less than 1EU per μg by the LAL method.
Supplied as 0.22μm filtered solution in PBS, 300mM NaCl (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized APC equivalent Human Peptide Ready HLA-A*02:01&B2M Tetramer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
Peptide Ready HLA-A*02:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
MHC; HLA-A*02:01; Peptide Ready
[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
[2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.
