MHC-HM44RTF-100μg / 询价
MHC-HM44RTF-500μg / 询价
MHC-HM44RTF-500μgx2 / 询价
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FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein
Recombinant FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*03:01) and Ile21-Met119 (B2M) [Accession | NP_002107.3(HLA-A*03:01)&P61769(B2M)].
The protein has a predicted MW of 301.2 kDa.
Excitation Wavelength: 490 nm
Emission Wavelength: 520 nm
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Anti-HLA class I (W6/32) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for FITC-equivalent Human Peptide Ready HLA-A*03:01&B2M Tetramer, His Tag with the EC50 of 2.55μg/ml determined by ELISA.
Peptide Ready HLA-A*03:01&B2M Tetramer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*03:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
MHC; HLA-A*03:01; Peptide Ready
[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
[2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.
