MHC-HM53RB-100μg / 询价
MHC-HM53RB-500μg / 询价
MHC-HM53RB-500μgx2 / 询价
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Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer Protein
Recombinant Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305(HLA-A*33:03) and Ile21-Met119(B2M) [Accession | VCU43110.1(HLA-A*33:03)&P61769(B2M)].
The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
>95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated Human Peptide Ready HLA-A*33:03&B2M Monomer, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test).
HLA-A*33:03&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A*33:03. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
MHC; HLA-A*33:03; Peptide Ready
[1] Moritz A, Anjanappa R, Wagner C, Bunk S, Hofmann M, Pszolla G, Saikia A, Garcia-Alai M, Meijers R, Rammensee HG, Springer S, Maurer D. High-throughput peptide-MHC complex generation and kinetic screenings of TCRs with peptide-receptive HLA-A*02:01 molecules. Sci Immunol. 2019 Jul 19;4(37):eaav0860.
[2] Sun Y, Young MC, Woodward CH, Danon JN, Truong H, Gupta S, Winters TJ, Burslem G, Sgourakis NG. Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. bioRxiv [Preprint]. 2023 Mar 18:2023.03.18.533266.
