MIC-HM40BB-100μg / 询价
MIC-HM40BB-500μg / 询价
MIC-HM40BB-500μgx2 / 询价
Biotinylated Human MICB Protein
Recombinant Biotinylated Human MICB Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala23-Gly298 [Accession | Q29980].
The protein has a predicted MW of 34.4 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human MICB on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Immobilized Biotinylated Human MICB, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA (QC Test).
Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43μg/ml determined by ELISA.
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
MICB; MIC-B; PERB11.2
(1)Spear P , Wu M R , Sentman M L , et al. NKG2D ligands as therapeutic targets[J]. Cancer immunity: a journal of the Academy of Cancer Immunology, 2013, 13(2):8.
