MSL-CM280-100μg / 询价
MSL-CM280-500μg / 询价
MSL-CM280-500μgx2 / 询价
Cynomolgus MSLN/Mesothelin Protein
Recombinant Cynomolgus MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Asp306-Gly590 [Accession | XP_065393170.1].
The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus MSLN, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 39.0ng/ml determined by ELISA (QC Test).
Immobilized Human CA125, His Tag at 5ug/ml (100μl/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA.
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
MSLN; CAK1 antigen; CAK1; Mesothelin; MPF; MPFSMRP; SMR;
(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.
