NEC-HM404 | 价格

NEC-HM404-100μg / 询价

NEC-HM404-500μg / 询价

NEC-HM404-500μgx2 / 询价

Non-biotinylated Human Nectin-4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus
It contains Gly32-Val351 [Accession | Q96NY8-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human Nectin-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Nectin-4 at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human Nectin-4, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 0.27 μg/ml determined by ELISA.

背景(Background)

NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life.

分子别名(Synonyms)

EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4

文献(References)

Chatterjee S, Sinha S, Kundu CN. Nectin cell adhesion molecule-4 (NECTIN-4): A potential target for cancer therapy. Eur J Pharmacol. 2021 Nov 15;911:174516. doi: 10.1016/j.ejphar.2021.174516. Epub 2021 Sep 20. PMID: 34547246.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.