NKC-HM495-100μg / 询价
NKC-HM495-500μg / 询价
NKC-HM495-500μgx2 / 询价
Human NKG2A&CD94 Protein
Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Arg100-Leu233 (NKG2A) and Ser34-Ile179 (CD94) [Accession | P26715-1(NKG2A)&Q13241-1(CD94)].
The protein has a predicted MW of 17.9 kDa (CD94) and 18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Immobilized Human NKG2A&CD94, His Tag at 1 μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-NKG2A Antibody, hFc Tag were added into Human NKG2A&CD94, His Tag : Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 31.8ng/ml.
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
(1) Hò GT, Celik AA, Huyton T, Hiemisch W, Blasczyk R, Simper GS, Bade-Doeding C. NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain. Int J Mol Sci. 2020 Jun 19;21(12):4362. doi: 10.3390/ijms21124362. PMID: 32575403; PMCID: PMC7352787.
