NKC-HM696B-100μg / 询价
NKC-HM696B-500μg / 询价
NKC-HM696B-500μgx2 / 询价
Biotinylated Human NKG2C&CD94 Protein
Recombinant Biotinylated Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Glu98-Leu231(NKG2C)&Ser34-Ile179(CD94) [Accession | P26717(NKG2C)&Q13241-1(CD94)].
The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human NKG2C&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.
NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.
NKG2C&CD94; NKG2C; CD94
[1] Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia. Int J Mol Sci. 2019 Mar 22;20(6):1454.
[2] Pump WC, Kraemer T, Huyton T, Hò GT, Blasczyk R, Bade-Doeding C. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8⁺ T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia. Int J Mol Sci. 2019 Mar 22;20(6):1454.
[3] López-Botet M, Muntasell A, Vilches C. The CD94/NKG2C+ NK-cell subset on the edge of innate and adaptive immunity to human cytomegalovirus infection. Semin Immunol. 2014 Apr;26(2):145-51.
