NSP-VE178-100μg / 询价
NSP-VE178-500μg / 询价
NSP-VE178-500μgx2 / 询价
表达区间及表达系统(Source)
|
SARS-COV-2 NSP7&NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7)&Ala1-Gln198(NSP8) [Accession | YP_009725303.1(NSP7)&YP_009725304.1(NSP8)]. |
分子量大小(Molecular Weight)
|
The protein has a predicted MW of 32.8 kDa same as Bis-Tris PAGE result. |
纯度(Purity)
|
> 95% as determined by Bis-Tris PAGE |
内毒素(Endotoxin)
|
Less than 1EU per μg by the LAL method. |
制剂(Formulation)
|
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution)
|
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储(Storage)
|
-20 to -80°C for 12 months as supplied from date of receipt. |
SARS-COV-2 NSP7&NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7)&Ala1-Gln198(NSP8) [Accession | YP_009725303.1(NSP7)&YP_009725304.1(NSP8)].
The protein has a predicted MW of 32.8 kDa same as Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM Nacl, 200mM Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The crystal structure of the metabolite of remdesivir (Monophosphate of GS-441524) and NSP12-NSP8-NSP7 of SARS CoV-2 virus was recently reported. The crystal structures of ADP-Ribose or AMP and NSP3 of SARS CoV-2 virus were also released, recently. The crystal structure of NSP3 of SARS CoV-2 virus as an alternative binding site of AMP or ADP-ribose to treat COVID-19.
SP7&NSP8; nsp7&nsp8
(1) Jung LS, Gund TM, Narayan M. Comparison of Binding Site of Remdesivir and Its Metabolites with NSP12-NSP7-NSP8, and NSP3 of SARS CoV-2 Virus and Alternative Potential Drugs for COVID-19 Treatment. Protein J. 2020 Dec;39(6):619-630. doi: 10.1007/s10930-020-09942-9. Epub 2020 Nov 13. PMID: 33185784; PMCID: PMC7662030.
NSP-VE178-100μg / 询价
NSP-VE178-500μg / 询价
NSP-VE178-500μgx2 / 询价
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