NTS-HM201 | 价格

NTS-HM201-100μg / 询价

NTS-HM201-500μg / 询价

NTS-HM201-500μgx2 / 询价

Human NTS1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human NTS1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Ser24-Leu163 [Accession | P30990].

分子量大小(Molecular Weight)

The protein has a predicted MW of 42.9 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human NTS1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human NTS1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human NTS1, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-NTS Antibody, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA.

背景(Background)

Crystal structures of neurotensin receptor subtype 1 (NTS1) allowed us to visualize the binding mode of the endogenous peptide hormone neurotensin and its pharmacologically active C-terminal fragment NT(8-13) within the orthosteric binding pocket of NTS1.

分子别名(Synonyms)

NT; NTR; NTR1; NTRH; NTRR; NTS1; NTSR1; NMN-125; NN; NT/N; NTS

文献(References)

(1) Kling RC, Burchardt C, Einsiedel J, Hübner H, Gmeiner P. Structure-based exploration of an allosteric binding pocket in the NTS1 receptor using bitopic NT(8-13) derivatives and molecular dynamics simulations. J Mol Model. 2019 Jun 17;25(7):193. doi: 10.1007/s00894-019-4064-x. PMID: 31209646.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.